Other

Part:BBa_K755001:Design

Designed by: Krystle McMinn   Group: iGEM12_GeorgiaState   (2012-10-11)


Standardized multiple cloning site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 11
    Illegal XbaI site found at 34
    Illegal SpeI site found at 49
    Illegal PstI site found at 71
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 11
    Illegal NheI site found at 84
    Illegal SpeI site found at 49
    Illegal PstI site found at 71
    Illegal NotI site found at 22
    Illegal NotI site found at 60
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 11
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 11
    Illegal XbaI site found at 34
    Illegal SpeI site found at 49
    Illegal PstI site found at 71
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 11
    Illegal XbaI site found at 34
    Illegal SpeI site found at 49
    Illegal PstI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We wanted to eliminate an existing Xba1 site, replacing it with the appropriate sequence of restriction sites necessary for an iGEM compatible MCS. In synthesizing the linker, it was important to add extra sequence at each end to ensure that the restriction enzymes would cut at both the 3' and 5' ends.


Source

synthesized sequenced based on iGEM standards

References